High-performance chromatographic method for the purification of tissue-type plasminogen activator.

نویسندگان

  • O Matsuo
  • Y Tanbara
  • K Okada
  • H Fukao
  • H Bando
  • T Sakai
چکیده

High-performance affinity chromatography was performed on five ligand-bound columns in an attempt to purify tissue-type plasminogen activator (t-PA), which is a glycoprotein with a high affinity for fibrin and also has two Kringle structures and finger-domain in its molecule. The five columns were concanavalin A-5PW, p-aminobenzamidine-5PW, imidinodiacetic acid-5PW, boric acid-5PW and lysine-5PW. All five were able to rapidly separate t-PA from contaminating proteins, with high resolution and recovery.

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عنوان ژورنال:
  • Journal of chromatography

دوره 369 2  شماره 

صفحات  -

تاریخ انتشار 1986